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KMID : 1134820070360091099
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 9 p.1099 ~ p.1105
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In vitro Cytotoxic Effect of Extracts from Styela plicata
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Lee Bo-Bae
Cha Mi-Ran
Park Hae-Ryong
Lee Seung-Cheol
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Abstract
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The present study describes the preliminary evaluation of the anticancer activity of Styela plicata. Freeze?dried S. plicata was extracted with methanol, ethanol, acetone, and water, and then anticancer effect of the extracts was measured by the MTT reduction assay and phase?contrast microscopy on the HT?29 human colon carcinoma cells. Among the extracts, acetone extract showed the highest anticancer activity. The cell proliferation rates markedly decreased by 94.0％ at the concentration of 500 μg/mL of acetone extract compared with control cells. The acetone extract was further fractionated with hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The HT?29 cells with hexane layer extract (250 μg/mL) decreased the cell viability to 5.1％ of untreated control. The growth of SW620, HeLa, and MCF?7 cells was decreased to about 10％, by the treatment of hexane layer extract 250 μg/mL. Theses results suggest extracts from S. plicata as possible natural cancer therapeutic material.
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KEYWORD
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anticancer activity, Styela plicata, extract, MTT reduction assay
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